Advances in Biochemical Engineering, Volume 9 by V. L. Yarovenko (auth.)

By V. L. Yarovenko (auth.)

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5 + d*) d*. (5) When the diameter of the oil droplets is much smaller than that of the cells, the specific growth rate can be derived from Eqs. (4) and (5) as S P -- Pmax " - - - - - - ~ , t3, * a-~,--~ where a 2 Kp pp"d~ 10,,/~ ' (6) Mechanism of Liquid Hydrocarbon Uptake by Microorganisms and Growth Kinetics 45 and it is assumed that the oil droplet size in the culture system is proportional to the droplet size in the equivalent nonculture system, as suggested by Calderbank [43], that is dp = ot d p o , (7) where ct = proportionality constant.

The effect of the antifoamer on the volumetric oxygen transfer coefficient is shown in Fig. 18. This figure indicates that the oxygen transfer rate is decreased by the antifoamer for concentrations up to 10 ppm but remains approximately constant for higher concentrations. The volumetric oxygen transfer coefficient in the culture filtrate with antifoamer was approximately equal to the value for the aqueous solution of the antifoamer, as shown in Fig. 17. Therefore, the culture used included the antifoamer.

19-21. The rates of acid production were proportional to the rates of cell growth, though a time lag in acid production was observed. Consequently, the growth rate could be estimated from the rate of acid production. Figure 22 shows the effects of operating conditions on the specific growth rate. The maximum specific growth rate was approximately the same under the three sets of operating conditions given in Figs. 11 h -z, and 0,13 h -~ , respectively, as shown in Fig. 22. From the above results it is concluded that these operating conditions, with respect to the oxygen supply, are adequate for the growth of C.

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