By Joost van Mameren, Gijs J. L. Wuite, Iddo Heller (auth.), Erwin J. G. Peterman, Gijs J. L. Wuite (eds.)
Life scientists think that existence is pushed, directed, and formed via biomolecules engaged on their very own or in live performance. it is just within the previous few many years that technological breakthroughs in delicate fluorescence microscopy and single-molecule manipulation recommendations have made it attainable to monitor and control unmarried biomolecules and degree their person homes. The methodologies awarded in Single Molecule ideas: tools and Protocols are being utilized increasingly more to the research of biologically correct molecules, comparable to DNA, DNA-binding proteins, and motor proteins, and have gotten usual in molecular biophysics, biochemistry, and molecular and mobile biology. the purpose of Single Molecule thoughts: equipment and Protocols is to supply a vast evaluate of single-molecule ways utilized to biomolecules at the foundation of transparent and concise protocols, together with an outstanding advent to the main primary single-molecule ideas, similar to optical tweezers, single-molecule fluorescence instruments, atomic strength microscopy, magnetic tweezers, and tethered particle movement. Written within the hugely winning Methods in Molecular Biology™ sequence layout, chapters include introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, quite simply reproducible laboratory protocols, and notes on troubleshooting and warding off identified pitfalls.
Authoritative and obtainable, Single Molecule concepts: tools and Protocols serves as a terrific advisor to scientists of all backgrounds and gives a vast and thorough evaluate of the intriguing and still-emerging box of single-molecule biology.